A simple microassay for interleukin-2 activity.

Journal article

A simple in vitro assay is described to quantitate human interleukin-2 (IL-2). It is based on the spontaneous' activation of human peripheral T cells in tissue culture and the subsequent expression of the IL-2 receptor. The results obtained with this assay agree with those of assays which utilise IL-2 dependent T cell clones. The method is highly sensitive, reproducible and easy to perform.

Authors: Ando, I; Beverley, P

• Journal: Journal of immunological methods
• Volume: 96
• Issue: 1
• Pages: 133-137
• Publication date: 1987-01-01
• DOI: 10.1016/0022-1759(87)90377-2
• EISSN: 1872-7905
• ISSN: 0022-1759
• Language: English
• Keywords: Antigens, Surface; Receptors, Interleukin-2; Microchemistry; Growth Substances; T-Lymphocytes; Recombinant Proteins; Phenotype; Lymphocyte Activation; Receptors, Immunologic; Antigens, Differentiation, T-Lymphocyte; Monocytes; Humans; Cells, Cultured; Phytohemagglutinins; Interleukin-2