Longitudinal analysis of CD8 T-cell responses to HIV and hepatitis C virus in a cohort of co-infected haemophiliacs.

Harcourt, G; Donfield, S; Gomperts, E; Daar, E; Goulder, P; Phillips, R; Klenerman, P

Journal article

Longitudinal analysis of CD8 T-cell responses to HIV and hepatitis C virus in a cohort of co-infected haemophiliacs.

Abstract:: OBJECTIVE: To investigate CD8 T-cell responses to HIV and hepatitis C virus (HCV) over time in a group of co-infected children with haemophilia to assess the influence of the virus infections on each other and on clinical outcome. DESIGN: The HIV and HCV CD8 T-cell response of HLA-A2 co-infected individuals in the cohort were analysed at two time points, looking at the frequency and phenotype of HIV-specific T cells and assessing overall responses to the two viruses. METHODS: Peripheral blood mononuclear cells (PBMC) from 72 HLA-A2 co-infected individuals were analysed using an HIV HLA-A2 tetramer and by IFN-gamma ELISpot using a panel of HIV and HCV antigens. PBMC from a group of 26 HLA-A2 HIV mono-infected adults were also analysed as a comparison. RESULTS: We identified two distinct patterns of response: some patients had a limited response to either virus whilst others made responses to a range of HIV epitopes. HCV responses were detected only in those who made multiple responses to HIV epitopes (P<0.0001). HCV infection had an influence on the phenotype of HIV-specific CD8 T cells, with a reduction in relative perforin and CD57 expression. Lack of functional or tetramer-positive HIV-specific T cells was associated with a decline in absolute CD4 T-cell counts between the time points (up to 7 years; P = 0.005). CONCLUSION: HCV infection has an impact on the phenotype of HIV-specific CD8 T cells. In this well-defined cohort, failure to maintain effective CD8 T-cell responses against HIV may contribute to disease progression.

Publication status:: Published

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APA Style

Harcourt, G., Donfield, S., Gomperts, E., Daar, E., Goulder, P., Phillips, R., & Klenerman, P. (2005). Longitudinal analysis of CD8 T-cell responses to HIV and hepatitis C virus in a cohort of co-infected haemophiliacs. AIDS (London, England), 19(11), 1135–1143.

MLA Style

Harcourt, G, et al. “Longitudinal Analysis of CD8 T-Cell Responses to HIV and Hepatitis C Virus in a Cohort of Co-Infected Haemophiliacs.” AIDS (London, England), vol. 19, no. 11, 2005, pp. 1135–43.

Chicago Style

Harcourt, G, S Donfield, E Gomperts, et al. 2005. “Longitudinal Analysis of CD8 T-Cell Responses to HIV and Hepatitis C Virus in a Cohort of Co-Infected Haemophiliacs.” AIDS (London, England) 19 (11): 1135–43.
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