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Journal article

Visualization and measurement of DNA methyltransferase activity in living cells

Abstract:

In this unit, a live-cell assay to measure DNA (cytosine-5) methyltransferase (MTase) activity at the single-cell level is described. This method takes advantage of the irreversible binding of enzymatically active MTases to genomic DNA substituted with the mechanism-based inhibitor 5-aza-2′-deoxycytidine (5-aza-dC). The procedure comprises incorporation of this nucleoside analog into DNA during replication and quantification of the time-dependentMTase immobilization by fluorescence recovery a...

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Journal:
Current Protocols in Cell Biology
Issue:
SUPPL. 39
Publication date:
2008-01-01
DOI:
EISSN:
1934-2616
ISSN:
1934-2500
Source identifiers:
310403
Language:
English
Keywords:
Pubs id:
pubs:310403
UUID:
uuid:08bb8850-797e-40a7-a2f5-f4d473c562ae
Local pid:
pubs:310403
Deposit date:
2012-12-19

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