Experimental determination of the radiation dose limit for cryocooled protein crystals.

Journal article

Radiation damage to cryocooled protein crystals during x-ray structure determination has become an inherent part of macromolecular diffraction data collection at third-generation synchrotrons. Generally, radiation damage is an undesirable component of the experiment and can result in erroneous structural detail in the final model. The characterization of radiation damage thus has become an important area for structural biologists. The calculated dose limit of 2 x 10(7) Gy for the diffracting ...

Expand abstract

Authors: Owen, R; Rudiño-Piñera, E; Garman, E

• Publication status: Published
• Journal: Proceedings of the National Academy of Sciences of the United States of America
• Volume: 103
• Issue: 13
• Pages: 4912-4917
• Publication date: 2006-03-01
• DOI: 10.1073/pnas.0600973103
• EISSN: 1091-6490
• ISSN: 0027-8424
• Language: English
• Keywords: Models, Molecular; Dose-Response Relationship, Radiation; Photons; Crystallography, X-Ray; Protein Structure, Tertiary; Ferritins; Electrons; Crystallization; Apoproteins