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A Plasmodium berghei reference line that constitutively expresses GFP at a high level throughout the complete life cycle.

Abstract:

Green fluorescent protein (GFP) is a well-established reporter protein for the examination of biological processes. This report describes a recombinant Plasmodium berghei, PbGFPCON, that constitutively expresses GFP in a growth responsive manner in its cytoplasm from a transgene that is integrated into the genome and controlled by the strong promoter from a P. berghei elongation factor-1alpha gene. All life cycle forms of PbGFPCON except for male gametes can be easily visualized by fluorescen...

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Journal:
Molecular and biochemical parasitology More from this journal
Volume:
137
Issue:
1
Pages:
23-33
Publication date:
2004-09-01
DOI:
EISSN:
1872-9428
ISSN:
0166-6851
Language:
English
Keywords:
Pubs id:
pubs:255755
UUID:
uuid:07384f4c-1dcd-4dae-95c8-617c2cb06931
Local pid:
pubs:255755
Source identifiers:
255755
Deposit date:
2012-12-19

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