Mapping irradiation hybrids to cosmid and yeast artificial chromosome libraries by direct hybridization of Alu-PCR products.

Journal article

A direct hybridization protocol is described for screening cosmid and yeast artificial chromosome libraries with pools of Alu-PCR products from somatic cell or irradiation hybrids. This method eliminates purification, cloning and analysis of each individual Alu-PCR product before library screening. A series of human X chromosome irradiation hybrids were mapped by this method, using a cosmid reference library for comparisons between overlapping hybrids to identify interesting clones for further analysis.

Authors: Monaco, A; Lam, V; Zehetner, G; Lennon, G; Douglas, C

• Publication status: Published
• Journal: Nucleic acids research
• Volume: 19
• Issue: 12
• Pages: 3315-3318
• Publication date: 1991-06-01
• DOI: 10.1093/nar/19.12.3315
• EISSN: 1362-4962
• ISSN: 0305-1048
• Language: English
• Keywords: Genome, Human; Humans; X Chromosome; Cloning, Molecular; Chromosomes, Fungal; Gene Library; Polymerase Chain Reaction; Repetitive Sequences, Nucleic Acid; Molecular Sequence Data; Cosmids; DNA; Chromosome Mapping; Base Sequence; Nucleic Acid Hybridization