Journal article icon

Journal article

Mapping irradiation hybrids to cosmid and yeast artificial chromosome libraries by direct hybridization of Alu-PCR products.

Abstract:
A direct hybridization protocol is described for screening cosmid and yeast artificial chromosome libraries with pools of Alu-PCR products from somatic cell or irradiation hybrids. This method eliminates purification, cloning and analysis of each individual Alu-PCR product before library screening. A series of human X chromosome irradiation hybrids were mapped by this method, using a cosmid reference library for comparisons between overlapping hybrids to identify interesting clones for further analysis.
Publication status:
Published

Actions


Access Document


Publisher copy:
10.1093/nar/19.12.3315

Authors


More by this author
Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Human Genetics Wt Centre
Role:
Author
Journal:
Nucleic acids research More from this journal
Volume:
19
Issue:
12
Pages:
3315-3318
Publication date:
1991-06-01
DOI:
EISSN:
1362-4962
ISSN:
0305-1048
Language:
English
Keywords:
Pubs id:
pubs:115642
UUID:
uuid:07216453-76b4-4041-87c9-fd9c480ff402
Local pid:
pubs:115642
Source identifiers:
115642
Deposit date:
2012-12-19

Terms of use


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP