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Journal article

Sharper low-power STED nanoscopy by time gating.

Abstract:
Applying pulsed excitation together with time-gated detection improves the fluorescence on-off contrast in continuous-wave stimulated emission depletion (CW-STED) microscopy, thus revealing finer details in fixed and living cells using moderate light intensities. This method also enables super-resolution fluorescence correlation spectroscopy with CW-STED beams, as demonstrated by quantifying the dynamics of labeled lipid molecules in the plasma membrane of living cells.
Publication status:
Published

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Publisher copy:
10.1038/nmeth.1624

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Journal:
Nature methods
Volume:
8
Issue:
7
Pages:
571-573
Publication date:
2011-07-05
DOI:
EISSN:
1548-7105
ISSN:
1548-7091
URN:
uuid:0711d38a-7ef9-4846-b9a8-2c17833ea7b8
Source identifiers:
222202
Local pid:
pubs:222202

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