Journal article

Sharper low-power STED nanoscopy by time gating.

Abstract:: Applying pulsed excitation together with time-gated detection improves the fluorescence on-off contrast in continuous-wave stimulated emission depletion (CW-STED) microscopy, thus revealing finer details in fixed and living cells using moderate light intensities. This method also enables super-resolution fluorescence correlation spectroscopy with CW-STED beams, as demonstrated by quantifying the dynamics of labeled lipid molecules in the plasma membrane of living cells.

Publication status:: Published

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APA Style

Vicidomini, G., Moneron, G., Han, K., Westphal, V., Ta, H., Reuss, M., Engelhardt, J., Eggeling, C., & Hell, S. (2011). Sharper low-power STED nanoscopy by time gating. Nature Methods, 8(7), 571–573.

MLA Style

Vicidomini, G, et al. “Sharper Low-Power STED Nanoscopy by Time Gating.” Nature Methods, vol. 8, no. 7, 2011, pp. 571–73.

Chicago Style

Vicidomini, G, G Moneron, K Han, et al. 2011. “Sharper Low-Power STED Nanoscopy by Time Gating.” Nature Methods 8 (7): 571–73.
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Publisher copy:: 10.1038/nmeth.1624

Authors

+ Vicidomini, G More by this author

Role:: Author

+ Moneron, G More by this author

Role:: Author

+ Han, K More by this author

Role:: Author

+ Westphal, V More by this author

Role:: Author

+ Ta, H More by this author

Role:: Author

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Journal:: Nature methods More from this journal
Volume:: 8
Issue:: 7
Pages:: 571-573
Publication date:: 2011-07-01
DOI:: 10.1038/nmeth.1624
EISSN:: 1548-7105
ISSN:: 1548-7091

Language:: English
Keywords:: Macropodidae

Cell Membrane

Light

Microscopy, Fluorescence

Cell Line

Animals

Lipids

Time Factors
Pubs id:: pubs:222202
UUID:: uuid:0711d38a-7ef9-4846-b9a8-2c17833ea7b8
Local pid:: pubs:222202
Source identifiers:: 222202
Deposit date:: 2012-12-19
ARK identifier:: ark:/29072/ora_0711d38a7ef94846b9a82c17833ea7b8

Terms of use

Copyright date:: 2011

Licence:: Terms and Conditions of Use for Oxford University Research Archive

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