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Journal article

Comparison of different fluorescence fluctuation methods for their use in FRET assays: monitoring a protease reaction.

Abstract:

We compare the accuracy of a variety of Fluorescence Fluctuation Spectroscopy (FFS) methods for the study of Förster Resonance Energy Transfer (FRET) assays. As an example, the cleavage of a doubly labeled, FRET-active peptide substrate by the protease Trypsin is monitored and analyzed using methods based on fluorescence intensity, Fluorescence Correlation Spectroscopy (FCS) and Fluorescence Intensity Distribution Analysis (FIDA). The presented fluorescence data are compared to High-Pressure ...

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Publication status:
Published

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Publisher copy:
10.2174/138920105774370571

Authors


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Institution:
University of Oxford
Division:
MSD
Department:
RDM
Sub department:
Weatherall Insti. of Molecular Medicine
Role:
Author
Journal:
Current pharmaceutical biotechnology More from this journal
Volume:
6
Issue:
5
Pages:
351-371
Publication date:
2005-10-01
DOI:
EISSN:
1873-4316
ISSN:
1389-2010
Language:
English
Keywords:
Pubs id:
pubs:222162
UUID:
uuid:06f009c9-c0ff-4407-a21e-56c939c62c8c
Local pid:
pubs:222162
Source identifiers:
222162
Deposit date:
2012-12-19

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