- Abstract:
-
Förster resonance energy transfer (FRET)-based reporters are important tools to study the spatiotemporal compartmentalization of cyclic adenosine monophosphate (cAMP) in living cells. To increase the spatial resolution of cAMP detection, new reporters with specific intracellular targeting have been developed. Therefore it has become critical to be able to appropriately compare the signals revealed by the different sensors. Here we illustrate a protocol to calibrate the response detected by di...
Expand abstract - Publisher copy:
- 10.1007/978-1-62703-622-1-5
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- Journal:
- Methods in Molecular Biology
- Volume:
- 1071
- Pages:
- 59-71
- Publication date:
- 2014-01-01
- DOI:
- ISSN:
-
1064-3745
- URN:
-
uuid:06e9c843-ebf4-4f98-bef8-f0b62aef178a
- Source identifiers:
-
436826
- Local pid:
- pubs:436826
- Language:
- English
- Keywords:
- Copyright date:
- 2014
Journal article
Analysis of compartmentalized cAMP: A method to compare signals from differently targeted FRET reporters
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