Journal article icon

Journal article

Analysis of compartmentalized cAMP: A method to compare signals from differently targeted FRET reporters

Abstract:

Förster resonance energy transfer (FRET)-based reporters are important tools to study the spatiotemporal compartmentalization of cyclic adenosine monophosphate (cAMP) in living cells. To increase the spatial resolution of cAMP detection, new reporters with specific intracellular targeting have been developed. Therefore it has become critical to be able to appropriately compare the signals revealed by the different sensors. Here we illustrate a protocol to calibrate the response detected by di...

Expand abstract

Actions


Access Document


Publisher copy:
10.1007/978-1-62703-622-1-5

Authors


Expand authors...
Journal:
Methods in Molecular Biology
Volume:
1071
Pages:
59-71
Publication date:
2014-01-01
DOI:
ISSN:
1064-3745
URN:
uuid:06e9c843-ebf4-4f98-bef8-f0b62aef178a
Source identifiers:
436826
Local pid:
pubs:436826

Terms of use


Metrics


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP