- Abstract:
-
This report demonstrates that solvent water relaxation measurements can be used for quantitative screening of ligand binding and for mechanistic investigations of enzymes containing paramagnetic metal centers by using conventional NMR instrumentation at high field. The method was exemplified using prolyl hydroxylase domain containing enzyme 2 (PHD2), a human enzyme involved in hypoxic sensing, with Mn(II) substituting for Fe(II) at the active site. K(D) values were determined for inhibitors t...
Expand abstract - Publication status:
- Published
- Publisher copy:
- 10.1021/jm901537q
-
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- Journal:
- Journal of medicinal chemistry
- Volume:
- 53
- Issue:
- 2
- Pages:
- 867-875
- Publication date:
- 2010-01-05
- DOI:
- EISSN:
-
1520-4804
- ISSN:
-
0022-2623
- URN:
-
uuid:06b24475-be74-40ae-bc3b-9d5135434da9
- Source identifiers:
-
35037
- Local pid:
- pubs:35037
- Copyright date:
- 2010
Journal article
Using NMR solvent water relaxation to investigate metalloenzyme-ligand binding interactions.
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