Journal article
Using NMR solvent water relaxation to investigate metalloenzyme-ligand binding interactions.
- Abstract:
-
This report demonstrates that solvent water relaxation measurements can be used for quantitative screening of ligand binding and for mechanistic investigations of enzymes containing paramagnetic metal centers by using conventional NMR instrumentation at high field. The method was exemplified using prolyl hydroxylase domain containing enzyme 2 (PHD2), a human enzyme involved in hypoxic sensing, with Mn(II) substituting for Fe(II) at the active site. K(D) values were determined for inhibitors t...
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- Publication status:
- Published
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Bibliographic Details
- Journal:
- Journal of medicinal chemistry
- Volume:
- 53
- Issue:
- 2
- Pages:
- 867-875
- Publication date:
- 2010-01-01
- DOI:
- EISSN:
-
1520-4804
- ISSN:
-
0022-2623
- Source identifiers:
-
35037
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:35037
- UUID:
-
uuid:06b24475-be74-40ae-bc3b-9d5135434da9
- Local pid:
- pubs:35037
- Deposit date:
- 2012-12-19
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- Copyright date:
- 2010
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