Nucleoid-Associated Proteins Affect Mutation Dynamics in E. coli in a Growth Phase-Specific Manner

Journal article

Left-hand panels: Mutability as a function of H-NS, IhfA, or IhfB occupancy for all possible transitions and transversions. Light green: sequence bound by the focal protein during mid- and/or late exponential phase but not later. Dark green: sequence bound by the focal protein during transition to stationary and/or stationary phase but not earlier. Blue: sequence not bound by the focal NAP throughout growth. Right-hand panels provide odds ratios along with 95% confidence intervals, where values in excess of 1 indicate higher mutability in exclusively early- versus exclusively late-bound sequence. Note that very few genomic regions are bound exclusively early (see Table S3). This applies to H-NS in particular so that odds ratio estimates are correspondingly noisy.

Authors: Warnecke, T; Supek, F; Lehner, B

• Publication status: Published
• Peer review status: Peer reviewed
• Publisher: Public Library of Science
• Journal: PLoS Computational Biology
• Volume: 8
• Issue: 12
• Pages: e1002846-e1002846
• Publication date: 2012-12-20
• DOI: 10.1371/journal.pcbi.1002846
• EISSN: 1553-7358
• ISSN: 1553-734X
• Language: English
• Keywords: DNA replication; DNA; Escherichia coli; Biology; Plasma protein binding; SeqA protein domain; Mutation; Genetics; Origin of replication; SOS response; DNA-binding protein; Transcription factor; Cell biology; Gene; DNA repair; Nucleoid