- Abstract:
-
This article describes the construction of a set of versatile expression vectors based on the In-Fusion cloning enzyme and their use for high-throughput cloning and expression screening. Modifications to commonly used vectors rendering them compatible with In-Fusion has produced a ligation-independent cloning system that is (1) insert sequence independent (2) capable of cloning large PCR fragments (3) efficient over a wide (20-fold) insert concentration range and (4) applicable to expression ...
Expand abstract - Publication status:
- Published
- Files:
-
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(pdf, 3.3MB)
-
- Publisher copy:
- 10.1093/nar/gkm047
-
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- Journal:
- Nucleic acids research
- Volume:
- 35
- Issue:
- 6
- Pages:
- e45
- Publication date:
- 2007-01-01
- DOI:
- EISSN:
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1362-4962
- ISSN:
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0305-1048
- URN:
-
uuid:06a9ab89-b069-48d5-a239-621f82fd4a3e
- Source identifiers:
-
5094
- Local pid:
- pubs:5094
- Copyright date:
- 2007
Journal article
A versatile ligation-independent cloning method suitable for high-throughput expression screening applications.
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