This article describes the construction of a set of versatile expression vectors based on the In-Fusion cloning enzyme and their use for high-throughput cloning and expression screening. Modifications to commonly used vectors rendering them compatible with In-Fusion has produced a ligation-independent cloning system that is (1) insert sequence independent (2) capable of cloning large PCR fragments (3) efficient over a wide (20-fold) insert concentration range and (4) applicable to expression ...Expand abstract
- Publication status:
- Copyright date:
A versatile ligation-independent cloning method suitable for high-throughput expression screening applications.
If you are the owner of this record, you can report an update to it here: Report update to this record