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Journal article

A versatile ligation-independent cloning method suitable for high-throughput expression screening applications.

Abstract:

This article describes the construction of a set of versatile expression vectors based on the In-Fusion cloning enzyme and their use for high-throughput cloning and expression screening. Modifications to commonly used vectors rendering them compatible with In-Fusion has produced a ligation-independent cloning system that is (1) insert sequence independent (2) capable of cloning large PCR fragments (3) efficient over a wide (20-fold) insert concentration range and (4) applicable to expression ...

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Publication status:
Published

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Publisher copy:
10.1093/nar/gkm047

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Journal:
Nucleic acids research
Volume:
35
Issue:
6
Pages:
e45
Publication date:
2007-01-01
DOI:
EISSN:
1362-4962
ISSN:
0305-1048
URN:
uuid:06a9ab89-b069-48d5-a239-621f82fd4a3e
Source identifiers:
5094
Local pid:
pubs:5094

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