Journal article
Xenopus oocytes as a heterologous expression system for studying ion channels with the patch-clamp technique.
- Abstract:
-
Oocytes from the Xenopus laevis represent one of the most widely used expression systems for functional characterization of ion channels. Their large size facilitates both injection of heterologous cRNA and subsequent electrophysiological recordings of ion channel currents. Furthermore, Xenopus oocytes translate cRNA very efficiently, resulting in the generation of a large number of ion channels in the plasma membrane. In this chapter, we outline methods for oocyte preparation and maintenance...
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- Publisher copy:
- 10.1007/978-1-59745-526-8_10
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Bibliographic Details
- Journal:
- Methods in molecular biology (Clifton, N.J.)
- Volume:
- 491
- Pages:
- 127-139
- Publication date:
- 2008-01-01
- DOI:
- EISSN:
-
1940-6029
- ISSN:
-
1064-3745
- Source identifiers:
-
267021
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:267021
- UUID:
-
uuid:06a2ee56-5b26-4eb4-8511-013af0b80133
- Local pid:
- pubs:267021
- Deposit date:
- 2013-11-16
Terms of use
- Copyright date:
- 2008
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