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Xenopus oocytes as a heterologous expression system for studying ion channels with the patch-clamp technique.

Abstract:

Oocytes from the Xenopus laevis represent one of the most widely used expression systems for functional characterization of ion channels. Their large size facilitates both injection of heterologous cRNA and subsequent electrophysiological recordings of ion channel currents. Furthermore, Xenopus oocytes translate cRNA very efficiently, resulting in the generation of a large number of ion channels in the plasma membrane. In this chapter, we outline methods for oocyte preparation and maintenance...

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Institution:
University of Oxford
Division:
MSD
Department:
Pharmacology
Role:
Author
Journal:
Methods in molecular biology (Clifton, N.J.)
Volume:
491
Pages:
127-139
Publication date:
2008-01-01
DOI:
EISSN:
1940-6029
ISSN:
1064-3745
Source identifiers:
267021
Language:
English
Keywords:
Pubs id:
pubs:267021
UUID:
uuid:06a2ee56-5b26-4eb4-8511-013af0b80133
Local pid:
pubs:267021
Deposit date:
2013-11-16

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