Successful attenuation of humoral immunity to viral capsid and transgenic protein following AAV-mediated gene transfer with a non-depleting CD4 antibody and cyclosporine.

Journal article

The ability of transient immunosuppression with a combination of a non-depleting anti-CD4 (NDCD4) antibody and cyclosporine (CyA) to abrogate immune reactivity to both adeno-associated viral vector (AAV) and its transgene product was evaluated. This combination of immunosuppressants resulted in a 20-fold reduction in the resulting anti-AAV8 antibody titres, to levels in naïve mice, following intravenous administration of 2 × 10(12) AAV8 vector particles per kg to immunocompetent mice. This al...

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Authors: McIntosh, J; Cochrane, M; Cobbold, S; Waldmann, H; Nathwani, SA

• Publication status: Published
• Journal: Gene therapy
• Volume: 19
• Issue: 1
• Pages: 78-85
• Publication date: 2012-01-01
• DOI: 10.1038/gt.2011.64
• EISSN: 1476-5462
• ISSN: 0969-7128
• Language: English
• Keywords: Gene Transfer Techniques; Mice, Inbred BALB C; Immunity, Humoral; Male; Capsid Proteins; Humans; Transgenes; Cyclosporine; Injections, Intravenous; Genetic Therapy; Mice, Inbred C57BL; Adenoviridae; Antibodies, Viral; Interferon-beta; Injections, Intramuscular; Genetic Vectors; Animals; Dependovirus; Immunosuppression; Recombinant Proteins; CD4-Positive T-Lymphocytes; Mice