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Structure determination of noncanonical RNA motifs guided by 1 H NMR chemical shifts

Abstract:

Structured noncoding RNAs underlie fundamental cellular processes, but determining their three-dimensional structures remains challenging. We demonstrate that integrating 1H NMR chemical shift data with Rosetta de novo modeling can be used to consistently determine high-resolution RNA structures. On a benchmark set of 23 noncanonical RNA motifs, including 11 'blind' targets, chemical-shift Rosetta for RNA (CS-Rosetta-RNA) recovered experimental structures with high accuracy (0.6-2.0 Å all-hea...

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Publisher copy:
10.1038/nmeth.2876

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Publisher:
Nature Publishing Group
Journal:
Nature Methods
Volume:
11
Issue:
4
Pages:
413-416
Publication date:
2014-01-01
DOI:
EISSN:
1548-7105
ISSN:
1548-7091
URN:
uuid:06919519-9954-4040-8d3d-50419bc6aeb6
Source identifiers:
462728
Local pid:
pubs:462728
Language:
English

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