Journal article
Adjustment of the ratio between collagenase class II and I improves islet isolation outcome.
- Abstract:
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BACKGROUND: Previous studies have clarified the distinct roles of collagenase class I (ccI) and class II (ccII) in enzymatic release of islets from pancreatic tissue. The present study sought to enhance the limited knowledge about the optimal ratio between collagenase classes. METHODS: Rat islets were isolated utilizing 0.4 DMC-U of neutral protease and 20 PZ-U of fractionated NB-1 collagenase recombined to obtain a ccII/I ratio of 0.5, 1.0, and 1.5. Quality control included assessment of yie...
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- Publisher copy:
- 10.1016/j.transproceed.2005.09.079
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Bibliographic Details
- Journal:
- Transplantation proceedings
- Volume:
- 37
- Issue:
- 8
- Pages:
- 3450-3451
- Publication date:
- 2005-10-01
- DOI:
- EISSN:
-
1873-2623
- ISSN:
-
0041-1345
- Source identifiers:
-
260163
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:260163
- UUID:
-
uuid:06575895-77fa-467c-aaa6-0def2f3087aa
- Local pid:
- pubs:260163
- Deposit date:
- 2012-12-19
Terms of use
- Copyright date:
- 2005
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