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Adjustment of the ratio between collagenase class II and I improves islet isolation outcome.

Abstract:

BACKGROUND: Previous studies have clarified the distinct roles of collagenase class I (ccI) and class II (ccII) in enzymatic release of islets from pancreatic tissue. The present study sought to enhance the limited knowledge about the optimal ratio between collagenase classes. METHODS: Rat islets were isolated utilizing 0.4 DMC-U of neutral protease and 20 PZ-U of fractionated NB-1 collagenase recombined to obtain a ccII/I ratio of 0.5, 1.0, and 1.5. Quality control included assessment of yie...

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Institution:
University of Oxford
Division:
MSD
Department:
Surgical Sciences
Role:
Author
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Journal:
Transplantation proceedings
Volume:
37
Issue:
8
Pages:
3450-3451
Publication date:
2005-10-01
DOI:
EISSN:
1873-2623
ISSN:
0041-1345
Source identifiers:
260163
Language:
English
Keywords:
Pubs id:
pubs:260163
UUID:
uuid:06575895-77fa-467c-aaa6-0def2f3087aa
Local pid:
pubs:260163
Deposit date:
2012-12-19

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