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The role of previously unmeasured organic acids in the pathogenesis of severe malaria

Abstract:

Introduction Severe falciparum malaria is commonly complicated by metabolic acidosis. Together with lactic acid (LA), other previously unmeasured acids have been implicated in the pathogenesis of falciparum malaria.

Methods In this prospective study, we characterised organic acids in adults with severe falciparum malaria in India and Bangladesh. Liquid chromatography-mass spectrometry was used to measure organic acids in plasma and urine. Patients were followed until recovery or death.

Results Patients with severe malaria (n=138), uncomplicated malaria (n=102), sepsis (n=32) and febrile encephalopathy (n=35) were included. Strong ion gap (mean±SD) was elevated in severe malaria (8.2 mEq/L±4.5) and severe sepsis (8.6 mEq/L±7.7) compared with uncomplicated malaria (6.0 mEq/L±5.1) and encephalopathy (6.6 mEq/L±4.7). Compared with uncomplicated malaria, severe malaria was characterised by elevated plasma LA, hydroxyphenyllactic acid (HPLA), α-hydroxybutyric acid and β-hydroxybutyric acid (all P<0.05). In urine, concentrations of methylmalonic, ethylmalonic and α-ketoglutaric acids were also elevated. Multivariate logistic regression showed that plasma HPLA was a strong independent predictor of death (odds ratio [OR] 3.5, 95 % confidence interval [CI] 1.6–7.5, P=0.001), comparable to LA (OR 3.5, 95 % CI 1.5–7.8, P=0.003) (combined area under the receiver operating characteristic curve 0.81).

Conclusions Newly identified acids, in addition to LA, are elevated in patients with severe malaria and are highly predictive of fatal outcome. Further characterisation of their sources and metabolic pathways is now needed.

Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1186/s13054-015-1023-5

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Grant:
Major Overseas Programme Core Grant number 089275/Z/09/Z


Publisher:
BioMed Central
Journal:
Critical Care More from this journal
Volume:
19
Issue:
1
Article number:
317
Publication date:
2015-12-01
Acceptance date:
2015-08-11
DOI:
EISSN:
1466-609X
ISSN:
1364-8535


Language:
English
Pubs id:
pubs:545500
UUID:
uuid:06192f30-b033-4226-80b6-2a40ddad3fed
Local pid:
pubs:545500
Source identifiers:
545500
Deposit date:
2016-03-31
ARK identifier:

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