CTD kinase associated with yeast RNA polymerase II initiation factor b.

Journal article

A kinase activity specific for the C-terminal repeat domain (CTD) of RNA polymerase II is associated with nearly homogeneous yeast general initiation factor b by three criteria: cofractionation on the basis of size and charge and coinactivation by mild heat treatment. The kinase phosphorylates the CTD at multiple sites in a processive manner. Factor b may possess a DNA-dependent ATPase activity as well. Both kinase and DNA-dependent ATPase activities exhibit the same nucleotide requirements a...

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Authors: Feaver, W; Gileadi, O; Li, Y; Kornberg, R

• Publication status: Published
• Journal: Cell
• Volume: 67
• Issue: 6
• Pages: 1223-1230
• Publication date: 1991-12-01
• DOI: 10.1016/0092-8674(91)90298-D
• EISSN: 1097-4172
• ISSN: 0092-8674
• Language: English
• Keywords: Amino Acid Sequence; Phosphorylation; Transcription, Genetic; DNA Helicases; Substrate Specificity; Protein Kinases; Saccharomyces cerevisiae; Molecular Sequence Data; Macromolecular Substances; Transcription Factors; Adenosine Triphosphatases; DNA Mutational Analysis; RNA Polymerase II