Technical advance: the DNA-binding activity of gal4 is inhibited by methylation of the gal4 binding site in plant chromatin.

Journal article

Derivatives of the Saccharomyces cerevisiae transcription factor Gal4 which act as effective transcription activators in yeast, Drosophila, mammalian cells and plant protoplasts are shown to direct expression from a GUS reporter construct when expressed in transgenic tobacco. However, in comparison to 35S-GUS controls, Gal4-mediated expression of the reporter gene was relatively weak and extremely variable. GUS expression was lost as plants matured and it was almost undetectable in most of th...

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Authors: Gälweiler, L; Conlan, R; Mader, P; Palme, K; Moore, I

• Journal: Plant journal : for cell and molecular biology
• Volume: 23
• Issue: 1
• Pages: 143-157
• Publication date: 2000-07-01
• DOI: 10.1046/j.1365-313x.2000.00805.x
• EISSN: 1365-313X
• ISSN: 0960-7412
• Language: English
• Keywords: Fungal Proteins; Saccharomyces cerevisiae Proteins; Transcription Factors; RNA, Messenger; Binding Sites; Glucuronidase; Enzyme Activation; Chromatin; Azacitidine; Protein Binding; DNA-Binding Proteins; DNA Methylation; Gene Expression Regulation, Plant; Transgenes