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Analysis of C. elegans intestinal gene expression and polyadenylation by fluorescence-activated nuclei sorting and 3'-end-seq.

Abstract:

Despite the many advantages of Caenorhabditis elegans, biochemical approaches to study tissue-specific gene expression in post-embryonic stages are challenging. Here, we report a novel experimental approach for efficient determination of tissue-specific transcriptomes involving the rapid release and purification of nuclei from major tissues of post-embryonic animals by fluorescence-activated nuclei sorting (FANS), followed by deep sequencing of linearly amplified 3'-end regions of transcripts...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1093/nar/gks282

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Institution:
University of Oxford
Department:
Oxford, MSD, Biochemistry
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Institution:
University of Oxford
Department:
Oxford, MSD, Pathology, Sir William Dunn School
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Institution:
University of Oxford
Department:
Oxford, MSD, Biochemistry
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Funding agency for:
Furger, A
Publisher:
Oxford University Press Publisher's website
Journal:
Nucleic acids research Journal website
Volume:
40
Issue:
13
Pages:
6304-6318
Publication date:
2012-07-05
DOI:
EISSN:
1362-4962
ISSN:
0305-1048
URN:
uuid:05d0a815-417f-4c14-8fe8-0e7a8f543b77
Source identifiers:
322669
Local pid:
pubs:322669

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