Analysis of C. elegans intestinal gene expression and polyadenylation by fluorescence-activated nuclei sorting and 3'-end-seq.
Despite the many advantages of Caenorhabditis elegans, biochemical approaches to study tissue-specific gene expression in post-embryonic stages are challenging. Here, we report a novel experimental approach for efficient determination of tissue-specific transcriptomes involving the rapid release and purification of nuclei from major tissues of post-embryonic animals by fluorescence-activated nuclei sorting (FANS), followed by deep sequencing of linearly amplified 3'-end regions of transcripts...Expand abstract
- Publication status:
- Peer review status:
- Peer reviewed
- Publisher's version
- Oxford University Press Publisher's website
- Nucleic acids research Journal website
- Publication date:
- Source identifiers:
- Local pid:
- Copyright holder:
- Haenni et al.
- Copyright date:
- Copyright © 2012 Haenni et al. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Views and Downloads
If you are the owner of this record, you can report an update to it here: Report update to this record