Structural basis for RIFIN-mediated activation of LILRB1 in malaria

Journal article

The Plasmodium species that cause malaria are obligate intracellular parasites, and disease symptoms occur as they replicate within human blood. Despite risking immune detection, the parasite delivers proteins that bind host receptors to infected erythrocyte surfaces. In the causative agent of the most deadly human malaria, Plasmodium falciparum, RIFINs form the largest erythrocyte surface protein family¹. Some RIFINs can bind inhibitory immune receptors, acting as targets for unusual antibodies containing a LAIR1 ectodomain^2–4, or as ligands for LILRB1⁵. RIFINs stimulate LILRB1 activation and signalling⁵, thereby potentially dampening human immune responses. To understand this process, we determined a structure of a RIFIN bound to LILRB1. We show that the RIFIN mimics the natural activating ligand of LILRB1, MHC class I, in its LILRB1-binding mode. A single RIFIN mutation disrupts the complex, blocks LILRB1 binding by all tested RIFINs and abolishes signalling in a reporter assay. In a supported lipid bilayer system, which mimics NK cell activation by antibody-dependent cell-mediated cytotoxicity, both RIFIN and MHC are recruited to the NK cell immunological synapse and reduce cell activation, as measured by perforin mobilisation. Therefore, LILRB1-binding RIFINs mimic the binding mode of the natural ligand of LILRB1 and suppress NK cell function.

Authors: Harrison, TE; Mørch, AM; Felce, JH; Sakoguchi, A; Reid, AJ

• Publication status: Published
• Peer review status: Peer reviewed
• Publisher: Nature Research
• Journal: Nature
• Volume: 587
• Pages: 309-312
• Place of publication: England
• Publication date: 2020-07-10
• Acceptance date: 2020-07-06
• DOI: 10.1038/s41586-020-2530-3
• EISSN: 1476-4687
• ISSN: 0028-0836
• Pmid: 32650338
• Language: English
• Keywords: FFR