Transcription termination and RNA degradation contribute to silencing of RNA polymerase II transcription within heterochromatin.

Journal article

Within the heterochromatin of budding yeast, RNA polymerase II (RNAPII) transcription is repressed by the Sir2 deacetylase. Although heterochromatic silencing is generally thought to be due to limited accessibility of the underlying DNA, there are several reports of RNAPII and basal transcription factors within silenced regions. Analysis of the rDNA array revealed cryptic RNAPII transcription within the "nontranscribed" spacer region. These transcripts are terminated by the Nrd1/Sen1 complex ...

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Authors: Vasiljeva, L; Kim, M; Terzi, N; Soares, L; Buratowski, S

• Publication status: Published
• Journal: Molecular cell
• Volume: 29
• Issue: 3
• Pages: 313-323
• Publication date: 2008-02-01
• DOI: 10.1016/j.molcel.2008.01.011
• EISSN: 1097-4164
• ISSN: 1097-2765
• Language: English
• Keywords: Saccharomyces cerevisiae; RNA Stability; Gene Expression Regulation, Fungal; Genes, Fungal; Genes, Reporter; Heterochromatin; Silent Information Regulator Proteins, Saccharomyces cerevisiae; RNA, Fungal; RNA Polymerase II; DNA, Ribosomal; Transcription, Genetic