In vivo imaging of matrix metalloproteinase 12 and matrix metalloproteinase 13 activities in the mouse model of collagen-induced arthritis.

Journal article

OBJECTIVE:

To develop enzyme-activatable Förster resonance energy transfer (FRET) substrate probes to detect matrix metalloproteinase 12 (MMP-12) and MMP-13 activities in vivo in mouse models of inflammatory arthritis.

METHODS:

Peptidic FRET probes activated by MMP-12 and MMP-13 were reverse designed from inhibitors selected from a phosphinic peptide inhibitor library. Selectivity of the probes was demonstrated in vitro using MMP-1, MMP-2, MMP-3, MMP-12, and MMP-13...

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Authors: Lim, N; Meinjohanns, E; Bou-Gharios, G; Gompels, L; Nuti, E

• Publication status: Published
• Peer review status: Peer reviewed
• Publisher: Wiley Publisher's website
• Journal: Arthritis and rheumatology (Hoboken, N.J.) Journal website
• Volume: 66
• Issue: 3
• Pages: 589-598
• Publication date: 2014-03-01
• DOI: 10.1002/art.38295
• EISSN: 2326-5205
• ISSN: 2326-5191
• Language: English
• Keywords: Synovial Membrane; Mice; Fluorescence Resonance Energy Transfer; Matrix Metalloproteinase 13; Matrix Metalloproteinase 12; Matrix Metalloproteinase Inhibitors; Diagnostic Imaging; Arthritis, Experimental; Animals