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A high-throughput gateway-compatible yeast one-hybrid screen to detect protein-DNA interactions.

Abstract:

In recent years, new techniques have spurred the discovery of cis-regulatory DNA elements. These stretches of noncoding DNA contain combinations of recognition sites to which transcription factors (TFs) bind, and in doing so, these TFs can activate or repress transcription. These protein-DNA interactions form the core of gene regulatory networks (GRNs) that are responsible for the differential gene expression that allow diversification of cell types, developmental programs, and responses to t...

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Institution:
University of Oxford
Division:
MSD
Department:
Physiology Anatomy & Genetics
Role:
Author
Journal:
Methods in molecular biology (Clifton, N.J.) More from this journal
Volume:
786
Pages:
335-355
Publication date:
2012-01-01
DOI:
EISSN:
1940-6029
ISSN:
1064-3745
Language:
English
Keywords:
Pubs id:
pubs:345812
UUID:
uuid:055ea750-b094-43de-8857-707777ce42b7
Local pid:
pubs:345812
Source identifiers:
345812
Deposit date:
2013-11-16

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