Properties of Bacillus cereus hemolysin II: a heptameric transmembrane pore.

Journal article

The gene encoding hemolysin II (HlyII) was amplified from Bacillus cereus genomic DNA and a truncated mutant, HlyII(DeltaCT), was constructed lacking the 94 amino acid extension at the C terminus. The proteins were produced in an E. coli cell-free in vitro transcription and translation system, and were shown to assemble into SDS-stable oligomers on rabbit erythrocyte membranes and liposomes. The hemolytic activity of HlyII was measured with rabbit erythrocytes yielding an HC(50) value of 1.64...

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Authors: Miles, G; Bayley, H; Cheley, S

• Publication status: Published
• Journal: Protein science : a publication of the Protein Society
• Volume: 11
• Issue: 7
• Pages: 1813-1824
• Publication date: 2002-07-01
• DOI: 10.1110/ps.0204002
• EISSN: 1469-896X
• ISSN: 0961-8368
• Language: English
• Keywords: Amino Acid Sequence; Forecasting; Sodium Dodecyl Sulfate; Ion Channels; Erythrocyte Membrane; Liposomes; Lipid Bilayers; Bacillus cereus; Bacterial Proteins; Hemolysin Proteins; Sequence Alignment; Molecular Sequence Data; Bacterial Toxins