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Kinetic analysis of matrix metalloproteinase activity using fluorogenic triple-helical substrates.

Abstract:

Matrix metalloproteinase (MMP) family members are involved in the physiological remodeling of tissues and embryonic development as well as pathological destruction of extracellular matrix components. To study the mechanisms of MMP action on collagenous substrates, we have constructed homotrimeric, fluorogenic triple-helical peptide (THP) models of the MMP-1 cleavage site in type II collagen. The substrates were designed to incorporate the fluorophore/quencher pair of (7-methoxycoumarin-4-yl)a...

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Publication status:
Published

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Publisher copy:
10.1021/bi0101190

Authors


Lauer-Fields, JL More by this author
Sritharan, T More by this author
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Institution:
University of Oxford
Department:
Oxford, MSD, NDORMS
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Journal:
Biochemistry
Volume:
40
Issue:
19
Pages:
5795-5803
Publication date:
2001-05-05
DOI:
EISSN:
1520-4995
ISSN:
0006-2960
URN:
uuid:05489755-5fb9-4ec1-9043-093b61e248f8
Source identifiers:
227431
Local pid:
pubs:227431

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