Anti-T cell monoclonal antibodies conjugated to ricin as potential reagents for human GVHD prophylaxis: effect on the generation of cytotoxic T cells in both peripheral blood and bone marrow.

Journal article

Three murine anti-human monoclonal antibodies broadly reactive with human T cells were conjugated to intact ricin toxin. The purgative effects of these immunotoxins (IT) on alloreactive T cells after in vitro pretreatment was tested in an in vitro assay measuring the generation of cytotoxic T cells (CTL). Pretreatment was performed in the presence of lactose to block the native binding of ricin, thus rendering IT antibody specific. Blocking studies with free antibody showed that selective tox...

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Authors: Quinones, R; Youle, R; Kersey, J; Zanjani, E; Azemove, S

• Journal: Journal of Immunology
• Volume: 132
• Issue: 2
• Pages: 678-683
• Publication date: 1984-02-01
• EISSN: 1550-6606
• ISSN: 0022-1767
• Language: English
• Keywords: Hematopoietic Stem Cells; Adult; Antibodies, Monoclonal; Cytotoxicity, Immunologic; Bone Marrow Cells; Lymphocyte Activation; Graft vs Host Disease; Bone Marrow; Ricin; T-Lymphocytes; Humans; T-Lymphocytes, Cytotoxic; Binding, Competitive; Mice; Animals