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The predator problem and PCR primers in molecular dietary analysis: swamped or silenced; depth or breadth?

Abstract:

Dietary metabarcoding has vastly improved our ability to analyse the diets of animals, but it is hampered by a plethora of technical limitations including potentially reduced data output due to the disproportionate amplification of the DNA of the focal predator, here termed “the predator problem”. We review the various methods commonly used to overcome this problem, from deeper sequencing to exclusion of predator DNA during PCR, and how they may interfere with increasingly common multipredato...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1111/1755-0998.13705

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Institution:
University of Oxford
Division:
MPLS
Department:
Biology
Role:
Author
Publisher:
Wiley
Journal:
Molecular Ecology Resources More from this journal
Volume:
23
Issue:
1
Pages:
41-51
Publication date:
2022-09-10
Acceptance date:
2022-08-24
DOI:
EISSN:
1755-0998
ISSN:
1755-098X
Language:
English
Keywords:
Pubs id:
1275410
Local pid:
pubs:1275410
Deposit date:
2022-08-24

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