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The pattern of localization of the putative oocyte activation factor, phospholipase Czeta, in uncapacitated, capacitated, and ionophore-treated human spermatozoa.

Abstract:

BACKGROUND: Recent studies suggest that in mammals, oocyte activation at fertilization is triggered by a sperm-specific phospholipase C, PLCzeta. We investigated PLCzeta localization in human spermatozoa. METHODS: A polyclonal antibody was generated against human PLCzeta and used in immunoblotting and immunofluorescence studies of ejaculated human sperm in uncapacitated and capacitated states. An ionophore was also used to induce the acrosome reaction in vitro. RESULTS: After verifying specif...

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Publication status:
Published

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Publisher copy:
10.1093/humrep/den280

Authors


More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Women's and Reproductive Health
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Pharmacology
Role:
Author
Journal:
Human reproduction (Oxford, England)
Volume:
23
Issue:
11
Pages:
2513-2522
Publication date:
2008-11-01
DOI:
EISSN:
1460-2350
ISSN:
0268-1161
Source identifiers:
185118
Language:
English
Keywords:
Pubs id:
pubs:185118
UUID:
uuid:04c2497d-11db-4a74-b2bd-4695f9688f06
Local pid:
pubs:185118
Deposit date:
2012-12-19

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