Structural impact of human and Escherichia coli biotin carboxyl carrier proteins on biotin attachment.

Journal article

Holocarboxylase synthetase (HCS, human) and BirA (Escherichia coli) are biotin protein ligases that catalyze the ATP-dependent attachment of biotin to apocarboxylases. Biotin attachment occurs on a highly conserved lysine residue within a consensus sequence (Ala/Val-Met-Lys-Met) that is found in carboxylases in most organisms. Numerous studies have indicated that HCS and BirA, as well as biotin protein ligases from other organisms, can attach biotin to apocarboxylases from different organisms...

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Authors: Healy, S; McDonald, M; Wu, X; Yue, W; Kochan, G

• Publication status: Published
• Journal: Biochemistry
• Volume: 49
• Issue: 22
• Pages: 4687-4694
• Publication date: 2010-06-01
• DOI: 10.1021/bi901612y
• EISSN: 1520-4995
• ISSN: 0006-2960
• Language: English
• Keywords: Methylmalonyl-CoA Decarboxylase; Protein Structure, Tertiary; Carrier Proteins; Fatty Acid Synthetase Complex, Type II; Protein Binding; Substrate Specificity; Acetyl-CoA Carboxylase; Protein Subunits; Peptide Fragments; Carbon-Nitrogen Ligases; Humans; Crystallography, X-Ray; Repressor Proteins; Escherichia coli Proteins