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Polypyrimidine tract-binding protein stimulates the poliovirus IRES by modulating eIF4G binding.

Abstract:

Tethered hydroxyl-radical probing has been used to determine the orientation of binding of polypyrimidine tract-binding protein (PTB) to the poliovirus type 1 (Mahoney) (PV-1(M)) internal ribosome entry site/segment (IRES)-the question of which RNA-binding domain (RBD) binds to which sites on the IRES. The results show that under conditions in which PTB strongly stimulates IRES activity, a single PTB is binding to the IRES, a finding which was confirmed by mass spectrometry of PTB/IRES comple...

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Publication status:
Published

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Publisher copy:
10.1038/emboj.2010.231
Journal:
EMBO journal More from this journal
Volume:
29
Issue:
21
Pages:
3710-3722
Publication date:
2010-11-01
DOI:
EISSN:
1460-2075
ISSN:
0261-4189

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