Pluripotency factors regulate definitive endoderm specification through eomesodermin.

Journal article

Understanding the molecular mechanisms controlling early cell fate decisions in mammals is a major objective toward the development of robust methods for the differentiation of human pluripotent stem cells into clinically relevant cell types. Here, we used human embryonic stem cells and mouse epiblast stem cells to study specification of definitive endoderm in vitro. Using a combination of whole-genome expression and chromatin immunoprecipitation (ChIP) deep sequencing (ChIP-seq) analyses, we...

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Authors: Teo, A; Arnold, S; Trotter, M; Brown, S; Ang, LT

• Journal: Genes and development
• Volume: 25
• Issue: 3
• Pages: 238-250
• Publication date: 2011-02-01
• DOI: 10.1101/gad.607311
• EISSN: 1549-5477
• ISSN: 0890-9369
• Language: English
• Keywords: Endoderm; Biological Markers; Octamer Transcription Factor-3; Homeodomain Proteins; T-Box Domain Proteins; Humans; Pluripotent Stem Cells; Cell Differentiation; Activins; Nodal Protein; SOXB1 Transcription Factors; Gene Regulatory Networks; Mice; Cell Line; Gene Expression Regulation, Developmental; Animals