Semi-automated microseeding of nanolitre crystallization experiments.

Journal article

A simple semi-automated microseeding procedure for nanolitre crystallization experiments is described. Firstly, a microseed stock solution is made from microcrystals using a Teflon bead. A dilution series of this microseed stock is then prepared and dispensed as 100 nl droplets into 96-well crystallization plates, facilitating the incorporation of seeding into high-throughput crystallization pipelines. This basic microseeding procedure has been modified to include additive-screening and cross-seeding methods. Five examples in which these techniques have been used successfully are described.

Authors: Walter, T; Mancini, E; Kadlec, J; Graham, S; Assenberg, R

• Publication status: Published
• Journal: Acta crystallographica. Section F, Structural biology and crystallization communications
• Volume: 64
• Issue: Pt 1
• Pages: 14-18
• Publication date: 2008-01-01
• DOI: 10.1107/s1744309107057260
• EISSN: 1744-3091
• ISSN: 1744-3091
• Language: English
• Keywords: Proteomics; Nanotechnology; Bacterial Proteins; Genomics; Viral Proteins; Automation; Escherichia coli Proteins; Models, Molecular; Molecular Conformation; Crystallography, X-Ray