Quantitation of antigen-specific CD8+ T-cell responses.

Conference item

Enumeration of antigen-specific CD8+ T-cells has traditionally been largely dependent on the use of techniques that require a period of expansion in vitro. However the recent emergence of methods that can be used directly ex vivo to quantify antigen-specific CD8+ T cells has prompted a re-evaluation of the magnitude of viral-specific cellular immune responses. The purpose of this review is to describe the commonly used methods currently available to quantify and characterise antigen-specific T-cells and then to focus in more detail on the use of tetrameric complexes of HLA/peptide to study the cellular immune response.

Authors: Ogg, G; Mcmichael, A

• Publication status: Published
• Journal: Immunology letters
• Volume: 66
• Issue: 1-3
• Pages: 77-80
• Publication date: 1999-03-01
• Event title: 2nd International Symposium on Immunological Correlates of Protection from HIV Infection and Disease
• DOI: 10.1016/s0165-2478(98)00161-8
• EISSN: 1879-0542
• ISSN: 0165-2478
• Keywords: Humans; CD8-Positive T-Lymphocytes; Animals