Quantification of the anti-influenza drug zanamivir in plasma using high-throughput HILIC-MS/MS.

Journal article

BACKGROUND: parenteral zanamivir is a promising drug for the treatment of severe influenza. However, quantification of this polar drug in biological matrices has traditionally been difficult and the methods developed have been relatively insensitive. RESULTS: a high-throughput bioanalytical method for the analysis of zanamivir in human plasma using SPE in the 96-well plate format and LC coupled to positive MS/MS has been developed and validated according to US FDA guidelines. The method uses 50 microl of plasma and covers a large working range from 1-50, 000 ng/ml with a LOD of 0.50 ng/ml. CONCLUSION: this new LC-MS/MS assay is more sensitive than previous methods despite using a small plasma volume sample. It is particularly suitable for clinical studies on both parenteral and inhaled zanamivir.

Authors: Lindegardh, N; Hanpithakpong, W; Kamanikom, B; Farrar, J; Hien, T

• Publication status: Published
• Journal: Bioanalysis
• Volume: 3
• Issue: 2
• Pages: 157-165
• Publication date: 2011-01-01
• DOI: 10.4155/bio.10.189
• EISSN: 1757-6199
• ISSN: 1757-6180
• Language: English
• Keywords: Zanamivir; Chromatography, Liquid; Plasma; Humans; Tandem Mass Spectrometry; Influenza, Human; Reference Standards; Antiviral Agents