Lucky imaging: improved localization accuracy for single molecule imaging.

Journal article

We apply the astronomical data-analysis technique, Lucky imaging, to improve resolution in single molecule fluorescence microscopy. We show that by selectively discarding data points from individual single-molecule trajectories, imaging resolution can be improved by a factor of 1.6 for individual fluorophores and up to 5.6 for more complex images. The method is illustrated using images of fluorescent dye molecules and quantum dots, and the in vivo imaging of fluorescently labeled linker for activation of T cells.

Authors: Cronin, B; de Wet, B; Wallace, M

• Publication status: Published
• Journal: Biophysical journal
• Volume: 96
• Issue: 7
• Pages: 2912-2917
• Publication date: 2009-04-01
• DOI: 10.1016/j.bpj.2008.12.3945
• EISSN: 1542-0086
• ISSN: 0006-3495
• Language: English
• Keywords: Image Processing, Computer-Assisted; Mice; T-Lymphocytes; Sensitivity and Specificity; Microscopy, Fluorescence; Cell Survival; Animals; Membrane Proteins; Cell Line, Tumor; Fluorescent Dyes; Phosphoproteins; Adaptor Proteins, Signal Transducing; Quantum Dots; Fluorescence