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Journal article

HLA-DR typing using DNA amplification by the polymerase chain reaction and sequential hybridization to sequence-specific oligonucleotide probes.

Abstract:

A series of sequence-specific oligonucleotides (SSOs) have been used to type alleles at the HLA-DRB1 locus. Genomic DNA was amplified to high copy number by the polymerase chain reaction (PCR) and hybridizations of the dot-blotted, amplified DNA to a series of 14 SSOs enabled the identification of the major specificities DR1-DRw14. Certain alleles (DR3 and DR4) could be rapidly and accurately identified by running the products of allele-specific amplification of genomic DNA on agarose gels. T...

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Publication status:
Published

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Publisher copy:
10.1007/bf00241635

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Institution:
University of Oxford
Department:
Oxford, MSD, NDORMS
Role:
Author
Journal:
Immunogenetics
Volume:
32
Issue:
6
Pages:
413-418
Publication date:
1990-01-01
DOI:
EISSN:
1432-1211
ISSN:
0093-7711
URN:
uuid:03d85cf2-b246-47fe-bee0-fdd83d85415e
Source identifiers:
108519
Local pid:
pubs:108519

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