- Abstract:
-
A series of sequence-specific oligonucleotides (SSOs) have been used to type alleles at the HLA-DRB1 locus. Genomic DNA was amplified to high copy number by the polymerase chain reaction (PCR) and hybridizations of the dot-blotted, amplified DNA to a series of 14 SSOs enabled the identification of the major specificities DR1-DRw14. Certain alleles (DR3 and DR4) could be rapidly and accurately identified by running the products of allele-specific amplification of genomic DNA on agarose gels. T...
Expand abstract - Publication status:
- Published
- Publisher copy:
- 10.1007/bf00241635
-
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- Journal:
- Immunogenetics
- Volume:
- 32
- Issue:
- 6
- Pages:
- 413-418
- Publication date:
- 1990-01-01
- DOI:
- EISSN:
-
1432-1211
- ISSN:
-
0093-7711
- URN:
-
uuid:03d85cf2-b246-47fe-bee0-fdd83d85415e
- Source identifiers:
-
108519
- Local pid:
- pubs:108519
- Copyright date:
- 1990
Journal article
HLA-DR typing using DNA amplification by the polymerase chain reaction and sequential hybridization to sequence-specific oligonucleotide probes.
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