Synthesis of alkyne- and azide-modified oligonucleotides and their cyclization by the CuAAC (click) reaction.

Journal article

The Cu(I)-catalyzed alkyne-azide cycloaddition (CuAAC) reaction has been used to synthesize cyclic mini-DNA duplexes. The reaction is carried out on 5'-alkyne-3'-azide-labeled hairpin loop oligonucleotides and proceeds in high yield under mild conditions in as little as 5 min. The resultant duplexes have very high thermal stability and their CD spectra are characteristic of normal B-DNA. Copyright 2008 by John Wiley and Sons, Inc.

Authors: El-Sagheer, A; Brown, T

• Journal: Current protocols in nucleic acid chemistry / edited by Serge L. Beaucage ... [et al.]
• Volume: Chapter 4
• Publication date: 2008-12-01
• EISSN: 1934-9289
• Language: English