TlpC, a novel chemotaxis protein in Rhodobacter sphaeroides, localizes to a discrete region in the cytoplasm.

Journal article

TlpC is encoded in the second chemotaxis operon of Rhodobacter sphaeroides. This protein shows some homology to membrane-spanning chemoreceptors of many bacterial species but, unlike these, is essential for R. sphaeroides chemotaxis to all compounds tested. Genomic replacement of tlpC with a C-terminal gfp fusion demonstrated that TlpC localized to a discrete cluster within the cytoplasm. Immunogold electron microscopy also showed that TlpC localized to a cytoplasmic electron-dense region. Co...

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Authors: Wadhams, G; Martin, A; Porter, S; Maddock, JR; Mantotta, J

• Publication status: Published
• Journal: Molecular microbiology
• Volume: 46
• Issue: 5
• Pages: 1211-1221
• Publication date: 2002-12-01
• DOI: 10.1046/j.1365-2958.2002.03252.x
• EISSN: 1365-2958
• ISSN: 0950-382X
• Language: English
• Keywords: Membrane Proteins; Chemotaxis; Immunohistochemistry; Cytoplasm; Recombinant Fusion Proteins; Rhodobacter sphaeroides; Gene Deletion; Green Fluorescent Proteins; Bacterial Proteins; Luminescent Proteins