NMD is essential for hematopoietic stem and progenitor cells and for eliminating by-products of programmed DNA rearrangements.

Journal article

Nonsense-mediated mRNA decay (NMD) is a post-transcriptional surveillance process that eliminates mRNAs containing premature termination codons (PTCs). NMD has been hypothesized to impact on several aspects of cellular function; however, its importance in the context of a mammalian organism has not been addressed in detail. Here we use mouse genetics to demonstrate that hematopoietic-specific deletion of Upf2, a core NMD factor, led to the rapid, complete, and lasting cell-autonomous extincti...

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Authors: Weischenfeldt, J; Damgaard, I; Bryder, D; Theilgaard-Mönch, K; Thoren, L

• Journal: Genes and development
• Volume: 22
• Issue: 10
• Pages: 1381-1396
• Publication date: 2008-05-01
• DOI: 10.1101/gad.468808
• EISSN: 1549-5477
• ISSN: 0890-9369
• Language: English
• Keywords: Animals; Cells, Cultured; Mice, Inbred C57BL; RNA Stability; Codon, Nonsense; Mice; Models, Biological; Carrier Proteins; Oligonucleotide Array Sequence Analysis; Sequence Deletion; Base Sequence; Hematopoietic Stem Cells; Gene Rearrangement; Lymphoid Progenitor Cells; Gene Expression Profiling; Mice, Transgenic; Humans