Journal article

Two methods of temperature control for single-molecule measurements.

Abstract:: Modern single-molecule biophysical experiments require high numerical aperture oil-immersion objectives in close contact with the sample. We introduce two methods of high numerical aperture temperature control which can be implemented on any microscope: objective temperature control using a ring-shaped Peltier device, and stage temperature control using a fluid flow cooling chip in close thermal contact with the sample. We demonstrate the efficacy of each system by showing the change in speed with temperature of two molecular motors, the bacterial flagellar motor and skeletal muscle myosin.

Publication status:: Published

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APA Style

Baker, M., Inoue, Y., Takeda, K., Ishijima, A., & Berry, R. (2011). Two methods of temperature control for single-molecule measurements. European Biophysics Journal : EBJ, 40(5), 651–660.

MLA Style

Baker, M, et al. “Two Methods of Temperature Control for Single-Molecule Measurements.” European Biophysics Journal : EBJ, vol. 40, no. 5, 2011, pp. 651–60.

Chicago Style

Baker, M, Y Inoue, K Takeda, A Ishijima, and R Berry. 2011. “Two Methods of Temperature Control for Single-Molecule Measurements.” European Biophysics Journal : EBJ 40 (5): 651–60.
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Publisher copy:: 10.1007/s00249-010-0667-y

Authors

+ Baker, M More by this author

Role:: Author

+ Inoue, Y More by this author

Role:: Author

+ Takeda, K More by this author

Role:: Author

+ Ishijima, A More by this author

Role:: Author

+ Berry, R More by this author

Institution:: University of Oxford
Division:: MPLS
Department:: Physics
Sub department:: Condensed Matter Physics
Role:: Author

Journal:: European biophysics journal : EBJ More from this journal
Volume:: 40
Issue:: 5
Pages:: 651-660
Publication date:: 2011-05-01
DOI:: 10.1007/s00249-010-0667-y
EISSN:: 1432-1017
ISSN:: 0175-7571

Language:: English
Keywords:: Escherichia coli

Calibration

Flagella

Temperature

Rabbits

Myosin Type II

Microscopy

Torque

Animals

Bacterial Proteins
Pubs id:: pubs:115753
UUID:: uuid:0346cf49-3830-4f85-9080-52ea1a250d47
Local pid:: pubs:115753
Source identifiers:: 115753
Deposit date:: 2012-12-19
ARK identifier:: ark:/29072/ora_0346cf4938304f85908052ea1a250d47

Terms of use

Copyright date:: 2011

Licence:: Terms and Conditions of Use for Oxford University Research Archive

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