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Template-directed oligonucleotide strand ligation, covalent intramolecular DNA circularization and catenation using click chemistry.

Abstract:
The copper-catalyzed azide-alkyne cycloaddition reaction has been used for the template-mediated chemical ligation of two oligonucleotide strands, one with a 5'-alkyne and the other with a 3'-azide, to produce a DNA strand with an unnatural backbone at the ligation point. A template-free click-ligation reaction has been used for the intramolecular circularization of a single stranded oligonucleotide which was used as a template for the synthesis of a covalently closed DNA catenane.
Publication status:
Published

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Publisher copy:
10.1021/ja070273v

Authors


El-Sagheer, A More by this author
Tumpane, J More by this author
Lincoln, P More by this author
Wilhelmsson, LM More by this author
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Journal:
Journal of the American Chemical Society
Volume:
129
Issue:
21
Pages:
6859-6864
Publication date:
2007-05-05
DOI:
EISSN:
1520-5126
ISSN:
0002-7863
URN:
uuid:03426667-1e57-43da-8a04-ce7b3e574c63
Source identifiers:
400028
Local pid:
pubs:400028

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