Comparative lipidomics profiling of human atherosclerotic plaques.
BACKGROUND: We sought to perform a systematic lipid analysis of atherosclerotic plaques using emerging mass spectrometry techniques. METHODS AND RESULTS: A chip-based robotic nanoelectrospray platform interfaced to a triple quadrupole mass spectrometer was adapted to analyze lipids in tissue sections and extracts from human endarterectomy specimens by shotgun lipidomics. Eighteen scans for different lipid classes plus additional scans for fatty acids resulted in the detection of 150 lipid spe...Expand abstract
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