Chemistry in living cells: detection of active proteasomes by a two-step labeling strategy.

Journal article

In vivo targeting of the proteasome: Probe 1 is a cell-permeable irreversible inhibitor that alkylates the active-site residues of the proteasome in a Michael fashion. After cell lysis, a biotin moiety is introduced by Staudinger ligation to yield construct 2. This strategy allows activity profiling of the catalytic activities of the proteasome in vivo.

Authors: Ovaa, H; van Swieten, P; Kessler, B; Leeuwenburgh, M; Fiebiger, E

• Publication status: Published
• Journal: Angewandte Chemie (International ed. in English)
• Volume: 42
• Issue: 31
• Pages: 3626-3629
• Publication date: 2003-08-01
• DOI: 10.1002/anie.200351314
• EISSN: 1521-3773
• ISSN: 1433-7851
• Language: English
• Keywords: Molecular Structure; Blotting, Western; Electrophoresis, Polyacrylamide Gel; Affinity Labels; Proteasome Endopeptidase Complex; Multienzyme Complexes; Humans; Enzyme Inhibitors; Cysteine Endopeptidases; Luminescent Measurements; Animals; Tumor Cells, Cultured