A simple method to cure established tumors by inflammatory killing of normal cells.

Journal article

We describe a simple technology used to cure an established metastatic disease. Intradermal injection of plasmid DNA encoding a transcriptionally targeted cytotoxic gene, along with hsp70, not only promoted tissue-specific, inflammatory killing of normal melanocytes, but also induced a CD8(+) T-cell-dependent, antigen-specific response in mice that eradicated systemically established B16 tumors. This CD8(+) T cell response was subsequently suppressed in vivo within a few days. The data demons...

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Authors: Daniels, G; Sanchez-Perez, L; Diaz, R; Kottke, T; Thompson, J

• Publication status: Published
• Journal: Nature biotechnology
• Volume: 22
• Issue: 9
• Pages: 1125-1132
• Publication date: 2004-09-01
• DOI: 10.1038/nbt1007
• EISSN: 1546-1696
• ISSN: 1087-0156
• Language: English
• Keywords: Antigens; Melanoma; CD8-Positive T-Lymphocytes; Treatment Outcome; Mice, Inbred C57BL; Immunization; Immunotherapy; Genetic Therapy; Mice; Survival Analysis; Melanocytes; Viral Proteins; Thymidine Kinase; Mice, Nude; Animals; Immunity, Innate