Human cardiosphere-derived cells from patients with chronic ischaemic heart disease can be routinely expanded from atrial but not epicardial ventricular biopsies.

Journal article

To investigate the effects of age and disease on endogenous cardiac progenitor cells, we obtained right atrial and left ventricular epicardial biopsies from patients (n = 22) with chronic ischaemic heart disease and measured doubling time and surface marker expression in explant- and cardiosphere-derived cells (EDCs, CDCs). EDCs could be expanded from all atrial biopsy samples, but sufficient cells for cardiosphere culture were obtained from only 8 of 22 ventricular biopsies. EDCs from both a...

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Authors: Chan, H; Meher Homji, Z; Gomes, R; Sweeney, D; Thomas, G

• Publication status: Published
• Journal: Journal of cardiovascular translational research
• Volume: 5
• Issue: 5
• Pages: 678-687
• Publication date: 2012-10-01
• DOI: 10.1007/s12265-012-9389-0
• EISSN: 1937-5395
• ISSN: 1937-5387
• Language: English
• Keywords: Biological Markers; Chronic Disease; Middle Aged; Severity of Illness Index; Aged; Humans; Adult; Cell Differentiation; Cell Proliferation; Male; Hypercholesterolemia; Biopsy; Stem Cells; Diabetes Mellitus; Flow Cytometry; Cell Culture Techniques; Heart Ventricles; Smoking; Proto-Oncogene Proteins c-kit; Pericardium; Cell Separation; Aged, 80 and over; Antigens, Thy-1; Heart Atria; Hypertension; Spheroids, Cellular; Female; Myocardial Ischemia