Journal article
Protein stabilisation by compatible solutes: effect of mannosylglycerate on unfolding thermodynamics and activity of ribonuclease A.
- Abstract:
-
Differential scanning calorimetry, optical spectroscopy, and activity measurements were used to investigate the effect of mannosylglycerate, a negatively charged osmolyte widely distributed among thermophilic and hyperthermophilic archaea and bacteria, on the thermal unfolding of ribonuclease A (RNase A). For comparison, assays in the presence of trehalose, a canonical solute in mesophiles, and potassium chloride were also carried out. A thermodynamic analysis was performed by using different...
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- Publisher copy:
- 10.1002/cbic.200300574
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Bibliographic Details
- Journal:
- Chembiochem : a European journal of chemical biology
- Volume:
- 4
- Issue:
- 8
- Pages:
- 734-741
- Publication date:
- 2003-08-01
- DOI:
- EISSN:
-
1439-7633
- ISSN:
-
1439-4227
- Source identifiers:
-
416745
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:416745
- UUID:
-
uuid:03029678-0c57-48f0-acd7-122dfc53780b
- Local pid:
- pubs:416745
- Deposit date:
- 2013-11-17
Terms of use
- Copyright date:
- 2003
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