Journal article icon

Journal article

Characterization of the CAMPATH-1 (CDw52) antigen: biochemical analysis and cDNA cloning reveal an unusually small peptide backbone.

Abstract:

The CAMPATH-1 (CDw52) antigen has been purified from human spleen. The antigenic epitope is heat stable but sensitive to mild alkali treatment. Experiments with phosphatidylinositol-specific phospholipase C indicate that it is anchored by a glycosylphosphatidylinositol (GPI) anchor. An N-terminal sequence of 11 amino acids was determined, followed by an abrupt stop. Using short overlapping mixed oligonucleotide primers, cDNA synthesized from the mRNA of a human B cell line was amplified by th...

Expand abstract
Publication status:
Published

Actions


Access Document


Publisher copy:
10.1002/eji.1830210714

Authors


More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Pathology Dunn School
Role:
Author
Journal:
European journal of immunology
Volume:
21
Issue:
7
Pages:
1677-1684
Publication date:
1991-07-05
DOI:
EISSN:
1521-4141
ISSN:
0014-2980
URN:
uuid:02bf0b1e-ffdc-42e9-8b35-95c7e176e38b
Source identifiers:
16743
Local pid:
pubs:16743

Terms of use


Metrics


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP