Distribution of functional HIV-specific CD8 T lymphocytes between blood and secondary lymphoid organs after 8-18 months of antiretroviral therapy in acutely infected patients.

Oxenius, A; Yerly, S; Ramirez, E; Phillips, R; Price, D; Perrin, L

Journal article

Distribution of functional HIV-specific CD8 T lymphocytes between blood and secondary lymphoid organs after 8-18 months of antiretroviral therapy in acutely infected patients.

Abstract:: OBJECTIVES: To assess whether drug-induced suppression of the plasma viral load is associated with selective differential distribution of virus-specific CD8 T cells between the blood and secondary lymphoid organs. METHODS: HIV-specific CD8 T lymphocyte responses were quantified in matched peripheral blood and lymph node samples from seven patients starting treatment shortly after infection, who received antiretroviral therapy (ART) for a median of 14 months. Cells recovered from samples were subjected to IFN-gamma ELISPOT analysis. A series of synthetic peptides corresponding to previously characterized cytotoxic T lymphocyte epitopes restricted by HLA I molecules present in each patient were used as antigens, together with appropriate positive and negative controls. RESULTS: HIV-specific CD8 T lymphocyte responses were found in six of the seven patients. The observed frequencies of HIV-specific CD8 T lymphocytes and the pattern of epitope recognition was identical within the two compartments. These results also confirm the observation that functional HIV-specific CD8 T cells are preserved on ART in most patients initiating treatment at the time of primary HIV-1 infection. CONCLUSION: This investigation demonstrated that patterns of antigenic immunodominance as well as frequencies of HIV-specific CD8 T lymphocytes are similar in blood and lymphoid tissue compartments in HIV-infected individuals. These findings support current approaches to the identification of HIV-specific CD8 T lymphocyte reactivity based on leukocytes isolated from blood even in patients with ART-induced suppression of viral load.

Publication status:: Published

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APA Style

Oxenius, A., Yerly, S., Ramirez, E., Phillips, R., Price, D., & Perrin, L. (2001). Distribution of functional HIV-specific CD8 T lymphocytes between blood and secondary lymphoid organs after 8-18 months of antiretroviral therapy in acutely infected patients. AIDS (London, England), 15(13), 1653–1656.

MLA Style

Oxenius, A, et al. “Distribution of Functional HIV-Specific CD8 T Lymphocytes between Blood and Secondary Lymphoid Organs after 8-18 Months of Antiretroviral Therapy in Acutely Infected Patients.” AIDS (London, England), vol. 15, no. 13, 2001, pp. 1653–56.

Chicago Style

Oxenius, A, S Yerly, E Ramirez, R Phillips, D Price, and L Perrin. 2001. “Distribution of Functional HIV-Specific CD8 T Lymphocytes between Blood and Secondary Lymphoid Organs after 8-18 Months of Antiretroviral Therapy in Acutely Infected Patients.” AIDS (London, England) 15 (13): 1653–56.
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