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Discovery and systematic characterization of risk variants and genes for coronary artery disease in over a million participants

Abstract:
The discovery of genetic loci associated with complex diseases has outpaced the elucidation of mechanisms of disease pathogenesis. Here we conducted a genome-wide association study (GWAS) for coronary artery disease (CAD) comprising 181,522 cases among 1,165,690 participants of predominantly European ancestry. We detected 241 associations, including 30 new loci. Cross-ancestry meta-analysis with a Japanese GWAS yielded 38 additional new loci. We prioritized likely causal variants using functionally informed fine-mapping, yielding 42 associations with less than five variants in the 95% credible set. Similarity-based clustering suggested roles for early developmental processes, cell cycle signaling and vascular cell migration and proliferation in the pathogenesis of CAD. We prioritized 220 candidate causal genes, combining eight complementary approaches, including 123 supported by three or more approaches. Using CRISPR–Cas9, we experimentally validated the effect of an enhancer in MYO9B, which appears to mediate CAD risk by regulating vascular cell motility. Our analysis identifies and systematically characterizes >250 risk loci for CAD to inform experimental interrogation of putative causal mechanisms for CAD.
Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1038/s41588-022-01233-6

Authors


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Role:
Author
ORCID:
0000-0003-3223-9131
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Institution:
University of Oxford
Division:
MSD
Department:
RDM
Sub department:
RDM Cardiovascular Medicine
Oxford college:
St John's College
Role:
Author
ORCID:
0000-0003-2307-4021
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Role:
Author
ORCID:
0000-0002-1691-9615
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Role:
Author
ORCID:
0000-0003-3153-1552

Contributors


Publisher:
Springer Nature
Journal:
Nature Genetics More from this journal
Volume:
54
Issue:
12
Pages:
1803-1815
Publication date:
2022-12-06
Acceptance date:
2022-10-17
DOI:
EISSN:
1546-1718
ISSN:
1061-4036
Pmid:
36474045


Language:
English
Keywords:
Pubs id:
1312193
Local pid:
pubs:1312193
Deposit date:
2023-02-23

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